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Ambiguity codes
Ambiquity codes are codes that mean any of the two or any of the three nucleotides.
Normally, purine (R, A or G) is complemented into pyrimidine (Y, C or T) and amino (M, C or A) is complemented into keto (K, T or G).
Differently, weak (W, A or T, pairs with the two hydrogen bonds) and strong (S, G or C, pairs with three hydrogen bonds) are normally not swapped during complementation, leaving the S and W codes unchanged instead. This is logical as both a nucleotide and its complement use the same number of bonds to make a complementing pair.
See this, for instance.
Here is the complete table of our converter:
| Code | Nucleotides | Complement |
|---|
| A | A | T |
| G | G | C |
| C | C | G |
| T | T | A |
| U | U | A |
| R | A or G, purine | Y, pyrimidine |
| Y | C or T, pyrimidine | R, purine |
| S | C or G, strong pairing | S, unchanged |
| W | A or T, weak pairing | W, unchanged |
| K | G or T, keto | M, amino |
| M | A or C, amino | K, keto |
| B | C or G or T, not A | V, not T |
| V | A or C or G, not T | B, not A |
| D | A or G or T, not C | H, not G |
| H | A or C or T, not G | D, not C |
| - | gap | unchanged |
| any other | any or unknown | unchanged |